Human osteopontin (OPN) enzyme-linked immunosorbent assay (ELISA) kit instruction manual

The kit uses a double antibody sandwich assay to determine the level of human osteopontin (OPN) in the specimen. The microporous plate was coated with purified human osteopontin (OPN) antibody to prepare a solid phase antibody, and osteopontin (OPN) was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled osteopontin (OPN). The antibody-antigen-enzyme-labeled antibody complex is formed by binding, and after thorough washing, the substrate TMB is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with osteopontin (OPN) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the human osteopontin (OPN) content in the sample was calculated from a standard curve.

The blush brush is a flat brush slightly smaller than the honey powder brush. The tops of the bristles are arranged in a semicircle.

A good blush brush can make rouge easy and natural. Dip the brush into blush powder, gently shake off the excess powder, and then apply makeup. If the color is not enough, slowly add it. Can also be used as a powder brush or after-painting

Blush Brushes

Blush Brushes,Morphe Blush Brush,Angled Blush Brush,Blush Makeup Brush

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